Molecular Biology Reagents and Kits
Apexbio Technology LLC ARCA Cy3 EGFP mRNA (5-moUTP), 100ug (1mg/mL)
ARCA Cy3 EGFP mRNA (5-moUTP) can be used to analyze mRNA delivery and translation efficiency. The EGFP mRNA will express an enhanced version of green fluorescent protein, which was originally isolated from the jellyfish, Aequorea victoria. EGFP is a direct detection reporter gene commonly used in mammalian cell cultures to produce bright green fluorescence with an emission peak at 509 nm. EGFP mRNA labeled with cyanine 3 can be directly visualized. ARCA Cy3 EGFP mRNA (5-moUTP) is an ideal molecule for determining mRNA delivery and localization and is independent of translation. Other sizes are also available. Please inqury us for quote.
GINKGO BIOWORKS INC (3P) Ginkgo Bioworks™ Cell Free Protein Synthesis - Economy kit (E. coli CFPS): 10 mL
Ginkgo’s cell free protein synthesis (CFPS) Economy kit is formulated to maximize protein expression per dollar spent. The E. coli transcription/translation system produces proteins from a T7 RNA polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification. The 10 mL kit enables user to run 500 reactions of 20 µL, or user can scale reaction volumes to their own specifications. Storage at -80° C. For research use only.
Meridian Life Science Gly-Free Phi29 Polymerase -HC, 100UL
Glycerol-Free Phi29 DNA Polymerase (HC) is a highly processive DNA-dependent DNA polymerase with strong strand displacement activity, which enables efficient isothermal DNA amplification from low DNA inputs. Glycerol-Free Phi29 DNA Polymerase (HC) 3′ - 5′ exonuclease activity delivers high fidelity amplification, making it an appropriate solution for sequencing DNA template preparation. It is supplied with a 5x Reaction Buffer that contains excipients required for lyophilization. Glycerol-Free Phi29 DNA Polymerase (HC) enables flexible and scalable reaction volumes, and is suitable for applications such as rolling circle amplification (RCA) and multiple displacement amplification (MDA).
Medchemexpress LLC Phi29 DNA polymerase | 125 U
Phi29 DNA polymerase is a protein-engineered Phi29 DNA polymerase expressed in E. coli, purified and isolated multiple times. It exhibits higher amplification efficiency and sensitivity compared to the wild-type enzyme, significantly shortening reaction times.
- High amplification efficiency and sensitivity: Greatly shortens reaction time.
- Special strand displacement activity: Possesses efficient continuous synthesis characteristics.
- Strong binding to templates: Can continuously synthesize DNA fragments up to 70kb without dissociating from the template.
- Strong 3′→5′ exonuclease proofreading function: Fidelity is 100 times higher than Taq DNA polymerase.
- High purity: Multiple column purifications ensure a clear single-purpose band in SDS-PAGE gel detection, with no E. coli DNA residue or endonuclease/exonuclease contamination in qPCR detection.
Enzo Life Sciences NUCLEOLAR-ID Green detection kit (1Kit)
Enzo Life Sciences’ NUCLEOLAR-ID Green Detection Kit contains a proprietary dye suitable for live-cell staining of nucleoli. The dye allows examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms. This kit is specifically designed for visualizing nucleoli in living cells. The dye in the kit is resistant to photobleaching, facilitating its use in imaging applications. This nucleolar dye may be used in conjunction with common live-cell nuclear counterstains such as Hoechst 33342, Draq5™, Vybrant DyeCycle™ Ruby and Enzo’s NUCLEAR-ID Red dye.Applications: Fluorescence microscopy, Fluorescent detection .
Enzo Life Sciences BIO-PROBE JC virus probe (2µg)
The JC Virus BIO-PROBE labeled probe is prepared by nick translation of a clone of the entire JC virus genome. JC virus, a member of the papovavirus family, usually causes subclinical infections. However, in immunocompromised individuals it can cause a fatal demyelinating brain disease, progressive multifocal leukoencephalopathy. JC virus is also neurooncogenic in primates. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis). Concentration: 20µg/ml. Formulation: Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA. Long Term Storage: -20°C.
Cayman Chemical albonIger PuromycIn 5mg
An inhibitor of protein synthesis; inhibits protein synthesis by 99% in vitro; an inhibitor of PSA and APN (IC50s = 9.7 and 41 µM, respectively); reduces viability of HL-60 and MOLT-4 cells (EC50s = 0.055 and 0.17 µM, respectively); active against the chloroquine-sensitive and -resistant P. falciparum strains T9-96 and K1 (IC50s = 0.024 and 0.023 µM, respectively); has been used as a selective marker in cell culture systems and has been chemically modified for use in labeling or imaging newly synthesized proteins
Diagnocine LLC NUCLEOSEEING-LIVE NUCLEUSGREEN
NucleoSeeing
Apexbio Technology LLC ARCA EGFP mRNA (5-moUTP), 5x1mg (1mg/mL)
EGFP (enhanced green fluorescent protein) mRNA can express an enhanced green fluorescence when transfected into cells, it is used as a direct-detection reporter mRNA with fluorescence emission at 509 nm. Our ARCA EGFP mRNA (5-moUTP) is provided at a concentration of 1mg/ml, it is ARCA capped, polyA-tailed and incorporated with modified nucleotides.Anti-Reverse Cap Analog (ARCA) is a cap analog used during in vitro transcription for the generation of capped transcripts. ARCA obtains the ability only inserting in the proper orientation, results in forming mRNAs that can be translated twice efficiently as those initiated with mCAP. Other sizes are also available. Please inqury us for quote.
Hampton Research HR2-117/Natrix 2 - 10 ml, tube format
HR2-117/Natrix 2 - 10 ml, tube format. Please note items from this supplier are non-returnable.